Long PCR

Dr. Duncan Clark duncan at genesys.demon.co.uk
Thu Oct 2 05:56:58 EST 1997


In article <3432715B.72BA at virginia.edu>, Rajesh Kumar
<rk6n at virginia.edu> writes
>I need to do a long PCR (~9 kb). Can somebody suggest me a suitable
>polymerase and a source of it ?

9kb of what, human xsomal, bacterial xsomal or lambda. It makes a lot of
difference as to how difficult it will be. Lambda is possible with just
Taq the others need a mix and possibly a lot of playing.

Duncan
-- 
The problem with being on the cutting edge is that you occasionally get 
sliced from time to time....

Duncan Clark
DNAmp Ltd.
TEl/FAX 01252376288
http://www.dnamp.com
http://www.genesys.demon.co.uk



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