Bryan L. Ford
fordb at bcc.orst.edu
Thu Oct 2 20:01:10 EST 1997
Dr. Duncan Clark wrote:
> In article <3432715B.72BA at virginia.edu>, Rajesh Kumar
> <rk6n at virginia.edu> writes
> >I need to do a long PCR (~9 kb). Can somebody suggest me a suitable
> >polymerase and a source of it ?
> 9kb of what, human xsomal, bacterial xsomal or lambda. It makes a lot of
> difference as to how difficult it will be. Lambda is possible with just
> Taq the others need a mix and possibly a lot of playing.
Yes, good point. Our own successful 9.6 Kb work was with trout total
genomic DNA, which has about the same complexity as human genomic DNA.
More information about the Methods