Long PCR

Bryan L. Ford fordb at bcc.orst.edu
Thu Oct 2 20:01:10 EST 1997


Dr. Duncan Clark wrote:
> 
> In article <3432715B.72BA at virginia.edu>, Rajesh Kumar
> <rk6n at virginia.edu> writes
> >I need to do a long PCR (~9 kb). Can somebody suggest me a suitable
> >polymerase and a source of it ?
> 
> 9kb of what, human xsomal, bacterial xsomal or lambda. It makes a lot of
> difference as to how difficult it will be. Lambda is possible with just
> Taq the others need a mix and possibly a lot of playing.

Duncan:

Yes, good point. Our own successful 9.6 Kb work was with trout total
genomic DNA, which has about the same complexity as human genomic DNA.

-Bryan




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