Long PCR

[daniel jacobs]

In article <3432715B.72BA at virginia.edu>, Rajesh Kumar <rk6n at virginia.edu> wrote:

> Hi,
> 
> I need to do a long PCR (~9 kb). Can somebody suggest me a suitable
> polymerase and a source of it ? How about using a mixture of two
> polymerases, say, Taq and Pfu ?
> 
> Any suggestion will be highly appreciated.
> 
> Rajesh

Try using a mixture of taq/pfu (10/1 unit) in your standard PCR buffer. I
use it all the time and it works, at least upto 12 kB.

Good luck

Daniel