Long PCR

daniel jacobs d.jacobs at unsw.edu.au
Thu Oct 2 21:15:16 EST 1997


In article <3432715B.72BA at virginia.edu>, Rajesh Kumar <rk6n at virginia.edu> wrote:

> Hi,
> 
> I need to do a long PCR (~9 kb). Can somebody suggest me a suitable
> polymerase and a source of it ? How about using a mixture of two
> polymerases, say, Taq and Pfu ?
> 
> Any suggestion will be highly appreciated.
> 
> Rajesh

Try using a mixture of taq/pfu (10/1 unit) in your standard PCR buffer. I
use it all the time and it works, at least upto 12 kB.

Good luck

Daniel



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