PCR with only one known primer?

[Paul N Hengen]

George Karkanias (karkania at aecom.yu.edu) wrote:

> Is there anyway to do a PCR rxn and get a product when you only know the
> sequence for one of the primers?
> 
> Thanks in advance.
> 
> George Karkanias
> Assistant Professor of Neuroscience

Yes. I know of a couple ways to do it. You should look
at this review for details about boomeranging...

@article{Hengen1995Septibs,
author = "P. N. Hengen",
title = "Methods and reagents - Vectorette, splinkerette,
and boomerang {DNA} amplification",
journal = "Trends in Biochemical Sciences",
volume = "20",
number = "9",
pages = "372-373",
month = "September",
year = "1995"}

ftp://ftp.ncifcrf.gov/pub/methods/TIBS/sep95.txt

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