RFI: vector with selection for hygromycin B resistance

[Koen De Smet]

Don Chen wrote:
> 
> Hi:
> 
> I am looking for a vector that carries a gene for hygromycin  B
> resistance.  Does anyone know of either commercial or academic
> source(s) for such a vector?  Thanks for any help you can offer.
> 
> don
> 
We use hygromycin B resistance markers on our shuttle vectors for 
cloning and expression in mycobacteria. Our vectors are all based on 
p16R1, described in Microbiology 140, 133-138 (1994).

Selection is with 250 ug/ml hyg in E.coli, 50-100 ug/ml in mycobacteria. 

I am not sure if there are any suitable sites to cut out the hyg gene 
only. Email me if you want more info, or if you want me to send you some 
plasmid.


Koen De Smet
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___     Department of Medical Microbiology																		___
___     Imperial College School of Medicine at St Mary's    ___
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