why BSA is need ed in some resriction enzymes reaction

[Dr. Duncan Clark]

In article <34351DD9.17B7 at ibm.net>, Philip Cheng <lsu0640 at ibm.net>
writes
>Hi, there,
>
>Does anyone know why BSA is needed in some restriction enzymes reaction?
>

Most RE's have specific activities in excess of 1,000,000 u/mg therefore
your 5u represents just 5ng of protein. BSA may help decrease the amount
of non-specific absorption of the RE to plastics or just stabilizes it
through increasing the general protein concn. in the reaction.

Duncan
-- 
The problem with being on the cutting edge is that you occasionally get 
sliced from time to time....

Duncan Clark
DNAmp Ltd.
TEl/FAX 01252376288
http://www.dnamp.com
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