BAC-to-BAC questions?

Peter pxpst2 at vms.cis.pitt.delet.edu
Mon Oct 6 17:20:48 EST 1997


In article <Pine.GSO.3.96.971006144404.11657A-100000 at chuma>, "Yi Zhang
(BIO)" <yzhang1 at chuma.cas.usf.edu> wrote:
> bac-Virus. However, I have to adapt the SF9 cells to serum-free medium
> before the transfection with the supplied cellFectin. I have some hard
> time to adapt Sf9 cells to the serum free medium (Grace's medium).

I used EXCELL media for growing the sf9 cells that expressed my protein
because serum activated my protein.  The cells would live and grow just
fine in it


Peter



More information about the Methods mailing list