tph at mrc-lmb.cam.ac.uk
Mon Oct 6 09:12:56 EST 1997
Matthias <matthias at molgen.biologie.uni-marburg.de> wrote:
> Dear readers,
> The ß-glucuronidase (GUS) is useful reporter of expression in our
> system. I have constructed several mutants and want to detect the
> enzyme activity. Unfortunately, these mutants show very weak expression.
> (We use x-gluc enzymatic assays) I am looking for an enzymatic substrat
> that more sensitive than x-gluc. As an alternative I tried several
> elisas, but failed. Is there anybody, who could send me a detailed
> protocol for elisa detection of the ß-glucuronidase.
> thank you very much.
> Matthias at molgen.biologie.uni-marburg.de
Have you tried MUG - 4-methylumbeliferryl b-D-glucuronide ? I seem to
remember that's what I used many years ago to follow import of GUS
labeled signal sequences into plant mitochondria. The product is
fluorescent under UV. See M5664 or M9130 in the Sigma Catalogue
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