Long PCR

Martin Jakt mjakt at hkusua.hku.hk
Tue Oct 7 01:28:19 EST 1997


Hi,
Just a small question, which company supplies, "very high quality nucleotides," 
are the big companies in general equivalent in these terms?
thanks,
> 
> Also use very high quality nucleotides. The presence of trace levels of
> dUTP is enough to stop long PCR above say 10kb on lambda by inhibiting
> the proof-reading component.

Martin Jakt
Dept. of Biochemistry
University of Hong Kong



More information about the Methods mailing list