PCR with only one known primer?

lader at ambion.com lader at ambion.com
Tue Oct 7 16:51:28 EST 1997


In article <3434ED75.5D1D9188 at aecom.yu.edu>,
  George Karkanias <karkania at aecom.yu.edu> wrote:
>
> Is there anyway to do a PCR rxn and get a product when you only know the
> sequence for one of the primers?
>

If is from a genome - cut with a 4 base cutter before amplification and
ligate on an adapter - now you know the sequence on the other end.

If it is an RNA then you are talking RACE or cRACE (do a medline search)

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