Quantitative RT PCR

lader at ambion.com lader at ambion.com
Tue Oct 7 16:41:43 EST 1997


In article <whc23-0710971118380001 at powercenter-604.physiol.cam.ac.uk>,
  whc23 at cus.cam.ac.uk (Dr.W.H. Colledge) wrote:
>
> Can anyone please provide information or protocol for what is considered
> the best method for quantitative RT PCR. Thanks

In Lieu of owning a $100,000 piece of hardware...

Assuming you are intereste in a single message: 1. design a robust pair
of primers 2. establish a robust rt-pcr protocol providing reasonable
yield of a single product 3. design a deletion construct and synthesize
an RNA competitor 4. Do competitive RT-PCR with the competitor added to
your RNA samples pre-rt.

well...you asked
Eric Lader elader at ambion.com
Senior Scientist, PCR Applications
Ambion, Inc

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