PCR

[Usha RAMAN]

Hi

I am trying to design primers (20-mer, both forward and reverse) close to
the cloning site in the original TA cloning vector. But they include a EcoR1
and BstX1 (forward) and EcoRV and EcoR1 (reverse) site. Will these
sites pose a problem during PCR. Any suggestions will be helpful.

Thanks 

Usha
raman at email.chop.edu