beta-actin primers

cfb7 at psu.edu cfb7 at psu.edu
Tue Oct 7 15:03:40 EST 1997


I need to design some primers to use for control amplification in
RT-PCR.  I would like to choose primers that will result in a 500-600
bp band from B-actin transcripts, but don't know much about B-actin. 
Has anyone else done this?  I don't want to spend the $$$ (~ $4 per
rxn) to purchase them from Stratagene.  

Christie Blackman          cfb7 at psu.edu
Department of Biochemistry and Molecular Biology
Penn State University
University Park, PA



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