Detection of 3H-labelled proteins

REW rewhlab at cbdn.ca
Wed Oct 8 17:49:51 EST 1997


Hi, 
I'm trying to detect tritium-labelled proteins in dried SDS-PAGE gels by 
autoradiography.   What's the best way to do this?  I've been told to soak 
the gels in 1M salicylate before drying then to expose to film at -80C.  
Question 1: Is there a more sensitive method?  Question 2:  Is it really 
necessary to expose the film at -80C?

Thanks for your help.

REW
REWHLAB at CBDN.ca 



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