Detection of 3H-labelled proteins
rewhlab at cbdn.ca
Wed Oct 8 17:49:51 EST 1997
I'm trying to detect tritium-labelled proteins in dried SDS-PAGE gels by
autoradiography. What's the best way to do this? I've been told to soak
the gels in 1M salicylate before drying then to expose to film at -80C.
Question 1: Is there a more sensitive method? Question 2: Is it really
necessary to expose the film at -80C?
Thanks for your help.
REWHLAB at CBDN.ca
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