which PCR machine?

[David J McGee]

I've used Idaho Technologies' Rapid Cycler with enormous success.  I
learned PCR this way, and now I'm in a new lab that does it the
"old-fashioned" way.  It takes 3 plus hours this way compared to 30 min or
less with Rapid Cycler.  And you don't have to wait very long for someone
to get done using Rapid Cycler.  And you use 1/12 th of the enzyme.  And
you use 1/20 of the primer amount.  It's fabulous.  You just have to get
use to the capllary tubes, which sometimes break if you're not careful.
It's easier using the non-scored capillary tubes (and much cheaper).  

Of course, no affiliation with the company.  Just a happy customer!

David

David J. McGee, Ph. D.
dmcge001 at umabnet.ab.umd.edu

Postdoctoral Fellow in Dr. Harry Mobley's lab

University of Maryland, Baltimore
Department of Microbiology and Immunology
13009 Bressler Research Building 
655 W. Baltimore St.
Baltimore, MD 21201

Lab Phone: 410-706-0466
Fax: 410-706-6751