why BSA is need ed in some resriction enzymes reaction

kang at msvax.mssm.edu kang at msvax.mssm.edu
Thu Oct 9 20:16:08 EST 1997


I think BSA works in at least following 2 ways.

1. Block the binding of RE onto the plastic surface
2. Block the digestion of enzyme by protease
3. binds and cleans out contaminated SDS in the soln.

--- and maybe others also -------
b

In article <owPidDAkUgN0EwGA at demon.co.uk>, "Dr. Duncan Clark" <duncan at genesys.demon.co.uk> writes:
>In article <34351DD9.17B7 at ibm.net>, Philip Cheng <lsu0640 at ibm.net>
>writes
>>Hi, there,
>>
>>Does anyone know why BSA is needed in some restriction enzymes reaction?
>>
>
>Most RE's have specific activities in excess of 1,000,000 u/mg therefore
>your 5u represents just 5ng of protein. BSA may help decrease the amount
>of non-specific absorption of the RE to plastics or just stabilizes it
>through increasing the general protein concn. in the reaction.
>
>Duncan
>-- 
>The problem with being on the cutting edge is that you occasionally get 
>sliced from time to time....
>
>Duncan Clark
>DNAmp Ltd.
>TEl/FAX 01252376288
>http://www.dnamp.com
>http://www.genesys.demon.co.uk



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