long PCR

xantho at isv.cnrs-gif.fr xantho at isv.cnrs-gif.fr
Thu Oct 9 10:54:53 EST 1997


Hi Franck,

I have used a primer of 54 nucleotides to add the FLAG epitope to our 
gene of interest. The homology with the target sequence was only 16 
nucleotides, so an overhang of about 38.

So good luck with your PCR,

Guido Van den Ackerveken



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