Clean DNA for Transfection
RJ.Nederhand at pg.tno.nl
Thu Oct 9 17:11:31 EST 1997
I want to transfect some reporter gene constructs (with a very weak
promoter) in HepG2 and Hela Cells. Here in the lab I was told I have
to use CsCl purified (plasmid)DNA for transfection. My questions are:
- Is it indeed necessary to use CsCl purified DNA for transfection?
- Where can I find a good, simple and reliable prototocol for CsCl
purification (Off course I know Maniatis)?
Any help would be appreciated.
Thanks in advance,
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