restriction of plasmids in JM109

Mathieu Ingouff mathieu.ingouff at sgen.slu.se
Thu Oct 9 12:55:51 EST 1997


I have recently transformed E. coli strain JM109 with a construct based
on pBI101.2 . Plasmid DNA from positive colonies refuses to cut in the
presence of ECOR1 or HIND III. Is this due to the hsdR17 genotype of
JM109. If so what is a suitable alternative host as I need to confirm
transformation before proceeding to large scale plasmid DNA isolation.

Thankyou in anticipation. 
-- 


____________________________________
  Mathieu Ingouff, PhD student
  SLU, Department of  Forest Genetics
  Genetikvägen 1B, Box 7027
  S-750 07 Uppsala, Sweden
 
  Email: mathieu.ingouff at sgen.slu.se
  fax: +46.18.67.32.79.
  voice: +46.18.67.32.36.
____________________________________



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