Clean DNA for Transfection
klenchin at facstaff.REMOVE_TO_REPLY.wisc.edu
Thu Oct 9 20:10:01 EST 1997
In article <343D5693.13D6 at pg.tno.nl>, RJ.Nederhand at pg.tno.nl wrote:
#I want to transfect some reporter gene constructs (with a very weak
#promoter) in HepG2 and Hela Cells. Here in the lab I was told I have
#to use CsCl purified (plasmid)DNA for transfection. My questions are:
#- Is it indeed necessary to use CsCl purified DNA for transfection?
Depends. Definitely not necessary if a factor of two is not a problem and
the *other* protocol works well. That *other* is the most variable thing,
#- Where can I find a good, simple and reliable prototocol for CsCl
#purification (Off course I know Maniatis)?
That's simple and reliable, isn't it?
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