Promega polyATract magnetic beads

David L. Haviland, Ph.D. dhavilan at IMM2.IMM.UTH.TMC.EDU
Fri Oct 10 13:41:44 EST 1997


At 18:24 10/10/97 +0100, Tim Hunt wrote:
>Promega insist that there is no  way to regenerate the streptavidin
>coated paramagnetic particles that are used in their kits for poly A
>purifications.  Has anybody had any joy with trying to regenerate these
>beads for re-use?

Tim:

As much as we suspect that companies hide reagents from us, Promega is
correct.  With an affinity of 10^-15, you can only irreverisbly break the
bond with either GTC or autoclaving.  Both of which might leave you
favorite protein or DNA worse for the wear.

Hope this helps,
David



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