Promega polyATract magnetic beads
David L. Haviland, Ph.D.
dhavilan at IMM2.IMM.UTH.TMC.EDU
Fri Oct 10 13:41:44 EST 1997
At 18:24 10/10/97 +0100, Tim Hunt wrote:
>Promega insist that there is no way to regenerate the streptavidin
>coated paramagnetic particles that are used in their kits for poly A
>purifications. Has anybody had any joy with trying to regenerate these
>beads for re-use?
As much as we suspect that companies hide reagents from us, Promega is
correct. With an affinity of 10^-15, you can only irreverisbly break the
bond with either GTC or autoclaving. Both of which might leave you
favorite protein or DNA worse for the wear.
Hope this helps,
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