restriction of plasmids in JM109

Dr. Duncan Clark duncan at genesys.demon.co.uk
Fri Oct 10 03:33:11 EST 1997


In article <343D1C1D.38D2 at sgen.slu.se>, Mathieu Ingouff
<mathieu.ingouff at sgen.slu.se> writes
>I have recently transformed E. coli strain JM109 with a construct based
>on pBI101.2 . Plasmid DNA from positive colonies refuses to cut in the
>presence of ECOR1 or HIND III. Is this due to the hsdR17 genotype of
>JM109.

No. HsdRM has no effect on EcoR I and Hind III re sites. Either you have
lost the sites in your construct or the plasmid prep is still not clean
enough. 

Duncan. 

-- 
The problem with being on the cutting edge is that you occasionally get 
sliced from time to time....

Duncan Clark
DNAmp Ltd.
TEl/FAX 01252376288
http://www.dnamp.com
http://www.genesys.demon.co.uk



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