restriction of plasmids in JM109

Dr. Duncan Clark duncan at
Fri Oct 10 03:33:11 EST 1997

In article <343D1C1D.38D2 at>, Mathieu Ingouff
<mathieu.ingouff at> writes
>I have recently transformed E. coli strain JM109 with a construct based
>on pBI101.2 . Plasmid DNA from positive colonies refuses to cut in the
>presence of ECOR1 or HIND III. Is this due to the hsdR17 genotype of

No. HsdRM has no effect on EcoR I and Hind III re sites. Either you have
lost the sites in your construct or the plasmid prep is still not clean


The problem with being on the cutting edge is that you occasionally get 
sliced from time to time....

Duncan Clark
DNAmp Ltd.
TEl/FAX 01252376288

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