LiCl precipitation of RNA at -20 degrees ?

Greg Nattrass gnattras at waite.adelaide.edu.au
Mon Oct 13 16:50:15 EST 1997


There is a FOCUS article from the mid 80's which showed that holding
nucleic acid samples at -20 prior to precipitation, actually does vey
little. It was found to be important only when precipitating low levels
of nucleic acid (ie low levels of cDNA) or when precipitating PCR
primers or very small DNA fragments. Another step which does very
little, is centrifuging samples at 4C. 

I routinely add glycogen to aid precipitation, such carriers produce a
visible pellet which  contains the DNA/RNA.  

Greg Nattrass



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