LiCl precipitation of RNA at -20 degrees ?

elader at ambion.com elader at ambion.com
Mon Oct 13 09:52:52 EST 1997


re: LiCl ppt of RNA

> It gets frozen. Does freezing help precipitation ? How does it compare to
precipitating at 4 degrees overnight ?

No, freezing does not help ppt. 2M LiCl will ppt RNA at 4 degrees, but I
have seen the -20 reference and also "on ice" i.e. 0 degrees. Maybe the
time the sample chills to the freezing point in the -20 is enough for
precipitation. Once frozen, pretty much nothing happens.

Eric

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