Dr. Duncan Clark
duncan at genesys.demon.co.uk
Sun Oct 12 04:07:20 EST 1997
In article <6154gl$hgb$1 at nargun.cc.uq.edu.au>, Paul Rohde
<P.Rohde at mailbox.uq.edu.au> writes
>Sorry for my ignorant responce, and I don't know if it is applicable
>to you, but there is a chemical method that transforms the methylated
>base from one type of base to another.
>So you PCR half your sample and sequence it.
>On the other sample, you treat it, PCR and sequence, then look
>for the base change differences (transformed bases).
Good idea, the only snag is that all PCR products are always
unmethylated unless you give the reaction methylated nucleotides. You
therefore automatically lose whatever methylation you started from.
The problem with being on the cutting edge is that you occasionally get
sliced from time to time....
More information about the Methods