Anti C-myc?

Fergus Doherty Fergus.Doherty at nottingham.ac.uk
Mon Oct 13 09:28:18 EST 1997


I have a CalBiochem/Oncogene Research Products mouse monoclonal anti c-myc
(OP10, clone 9E10) that has started to misbehave.  It binds to ALL proteins
on  a blot.  The samples are yeast transformants expressing a c-myc
epiptope tagged protein.  Anyone any clues as to why this has started to
happen?  I don't see this with exactly the same samples but a different
primary antibody.

Anyone suggest a CHEAP alternative source?  A polyclonal will do, must must
be able to recognise EQKLISEEDL.

Thanks

-- 
Fergus Doherty,
Dept Biochemistry,
Nottingham University,

Fergus.Doherty at nottingham.ac.uk
0115 970 9366 (74-41366 internal)



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