Heparitinase-Digest

Mathias DREXLER drexler at mol.univie.ac.at
Mon Oct 13 12:31:25 EST 1997


SOS-who can help me?

I´m doing Heparitinase-digests ( Heparitinase III from SIGMA) of different
Heparansulfatproteoglycans . But my proteins of interest are always caught
between the interface of the stacking gel and separating gel, and they do
not run into the gel even if i did the digest.So i think that the digest
is not working. So how can i check if the digest has worked.

Hoping for some ideas, i wish you all a nice day, Andy



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