Heparitinase-Digest

[Mathias DREXLER]

SOS-who can help me?

I´m doing Heparitinase-digests ( Heparitinase III from SIGMA) of different
Heparansulfatproteoglycans . But my proteins of interest are always caught
between the interface of the stacking gel and separating gel, and they do
not run into the gel even if i did the digest.So i think that the digest
is not working. So how can i check if the digest has worked.

Hoping for some ideas, i wish you all a nice day, Andy