3' overhang digestion
dspinella at chugaibio.com
Tue Oct 14 17:44:06 EST 1997
Javier Elbio Irazoqui <jei at acpub.duke.edu writes:
> I'm in need of a protocol for digestion of 3' overhangs. I don't have
> mung bean nuclease, but I do have S1 nuclease. The overhang is 2 bp long.
> I need to do this to clone this digestion fragment.
Why not just use Klenow or T4 polymerase and let the 3'->5' exo activity
do the job for you? Much safer than S1 nuclease which is likely to chew
back past the double-stranded portion of your fragment. Good Luck. --
More information about the Methods