3' overhang digestion

Dom Spinella dspinella at chugaibio.com
Tue Oct 14 17:44:06 EST 1997


Javier Elbio Irazoqui <jei at acpub.duke.edu writes:

> I'm in need of a protocol for digestion of 3' overhangs. I don't have 
> mung bean nuclease, but I do have S1 nuclease. The overhang is 2 bp long. 
> I need to do this to clone this digestion fragment.
> Thanx

Why not just use Klenow or T4 polymerase and let the 3'->5' exo activity
do the job for you?  Much safer than S1 nuclease which is likely to chew
back past the double-stranded portion of your fragment.  Good Luck.  --
Dom Spinella



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