AflIII site in pUC

[Nicolas Escriou]

Hello,

the AflIII site in a pUC18 derivative bothers me for cloning purposes. I
would like to destroy it by restriction-filling in-religation. Since this
site is close to colE1 origin of replication (61 bases downstream), I
would like to know if such a mutation could hamper pUC replication. Any
idea ?

Thanks in advance,

Nicolas

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Dr. Nicolas Escriou (escriou at pasteur.fr)
Unité de Génétique Moléculaire des Virus Respiratoires
Institut Pasteur
25, rue du Dr Roux
75724 Paris cedex 15
France
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