AflIII site in pUC

Nicolas Escriou escriou at
Tue Oct 14 08:33:51 EST 1997


the AflIII site in a pUC18 derivative bothers me for cloning purposes. I
would like to destroy it by restriction-filling in-religation. Since this
site is close to colE1 origin of replication (61 bases downstream), I
would like to know if such a mutation could hamper pUC replication. Any
idea ?

Thanks in advance,


Dr. Nicolas Escriou (escriou at
Unité de Génétique Moléculaire des Virus Respiratoires
Institut Pasteur
25, rue du Dr Roux
75724 Paris cedex 15

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