Peptide-KLH coupling HELP!!
Jeffrey Andrew Mahr
jmahr at emory.edu
Tue Oct 14 15:39:04 EST 1997
I am coupling a peptide to KLH using glutaraldehyde. When I stop the
reaction using 1M glycine (in PBS), the solution turns yellow, as it
should - but then turns orange. By the end of an hour it has turned
almost brown. I have coupled this same peptide (same synthesis) to KLH
before and the solution stayed yellow. Has anybody any light to shed on
this situation? I have tried twice, checking my pH's in between, and
gotten the same result.
THanks, Jeff Mahr jmahr at emory.edu
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