AflIII site in pUC

Dr. Duncan Clark duncan at
Wed Oct 15 03:40:28 EST 1997

In article <61vsbv$a9l$1 at>, Nicolas Escriou
<escriou at> writes
>the AflIII site in a pUC18 derivative bothers me for cloning purposes. I
>would like to destroy it by restriction-filling in-religation. Since this
>site is close to colE1 origin of replication (61 bases downstream), I
>would like to know if such a mutation could hamper pUC replication. Any
>idea ?

We have cloned into this site a few times without disrupting the colE1

The problem with being on the cutting edge is that you occasionally get 
sliced from time to time....

Duncan Clark
DNAmp Ltd.
TEl/FAX 01252376288

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