which amino acid mimicking phosphotyrosine?

[Andrey S. Shaw]

Lauge Schaffer wrote:
> 
> hammer at liai.org wrote:
> > Hi netters!
> > I would like to make a constitutive active mutant of a protein which
> > is activared by tyrosine phosphorylation.
> > I am thinking of substituting the the tyrosine residue by aspartic
> > acid. Is aspartic acid mimicking phosphotyrosine?
> > Are there any references?
> > Any hint will be appreciated,
> > thanks,
> > Stephan
> 
> Partial constitutive activation of the insulin receptor has been
> achieved in Asp and Glu mutations.
> 
> B. Zhang, J. M. Tavaré, L. Ellis, and R. A. Roth. The Regulatory Role of
> Known Tyrosine Autophosphorylation Sites of the Insulin Receptor Kinase
> Domain. J.Biol.Chem. 266:990-996, 1991.
> 
> Hope this helps,
> 
> Lauge Schäffer

Most of the time, it does not work.  It is always worth a try, but there
is no rule.


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