Tris buffer pH

Alexander N. Kukushkin kan at mmcd.cyt.ras.spb.ru
Wed Oct 15 07:11:10 EST 1997


>   Does anyone have a table which gives the relative amounts (mass) of
>   Tris-base and Tris-HCl to add to give a 1M solution of various different
>   pH values i.e. 7.4, 8.5 etc.? I am sick of adding a bucket-load of conc.
>   HCl to my 1M Tris-base solution to bring it down to 7.4. I know such
>   biological data tables exist, but as yet I have been unable to locate
>   them. Thank you in advance.
>
>   Warren Hirst
>
Among other things, such table is in the book: Dawson R.M.C. et al, Data for
Biochemical Research, Clarendon Press, Oxford, 1986.
Alex



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