Tris buffer pH

Cornelius Krasel krasel at wpxx02.toxi.uni-wuerzburg.de
Wed Oct 15 06:16:12 EST 1997


Dr Warren Hirst (w.hirst at ic.ac.uk) wrote:
> Does anyone have a table which gives the relative amounts (mass) of 
> Tris-base and Tris-HCl to add to give a 1M solution of various different 
> pH values i.e. 7.4, 8.5 etc.? I am sick of adding a bucket-load of conc. 
> HCl to my 1M Tris-base solution to bring it down to 7.4.

Why don't you calculate the amount of HCl you need to bring the pH to
the desired value. (Henderson-Hasselbalch does the trick.)

--Cornelius.

-- 
/* Cornelius Krasel, U Wuerzburg, Dept. of Pharmacology, Versbacher Str. 9 */
/* D-97078 Wuerzburg, Germany   email: phak004 at rzbox.uni-wuerzburg.de  SP4 */
/* "Science is the game we play with God to find out what His rules are."  */



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