Tris buffer pH

[Cornelius Krasel]

Dr Warren Hirst (w.hirst at ic.ac.uk) wrote:
> Does anyone have a table which gives the relative amounts (mass) of 
> Tris-base and Tris-HCl to add to give a 1M solution of various different 
> pH values i.e. 7.4, 8.5 etc.? I am sick of adding a bucket-load of conc. 
> HCl to my 1M Tris-base solution to bring it down to 7.4.

Why don't you calculate the amount of HCl you need to bring the pH to
the desired value. (Henderson-Hasselbalch does the trick.)

--Cornelius.

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/* Cornelius Krasel, U Wuerzburg, Dept. of Pharmacology, Versbacher Str. 9 */
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