Tris buffer pH
krasel at wpxx02.toxi.uni-wuerzburg.de
Wed Oct 15 06:16:12 EST 1997
Dr Warren Hirst (w.hirst at ic.ac.uk) wrote:
> Does anyone have a table which gives the relative amounts (mass) of
> Tris-base and Tris-HCl to add to give a 1M solution of various different
> pH values i.e. 7.4, 8.5 etc.? I am sick of adding a bucket-load of conc.
> HCl to my 1M Tris-base solution to bring it down to 7.4.
Why don't you calculate the amount of HCl you need to bring the pH to
the desired value. (Henderson-Hasselbalch does the trick.)
/* Cornelius Krasel, U Wuerzburg, Dept. of Pharmacology, Versbacher Str. 9 */
/* D-97078 Wuerzburg, Germany email: phak004 at rzbox.uni-wuerzburg.de SP4 */
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