Tris buffer pH

Jay Miller jhmiller at helix.nih.gov
Wed Oct 15 08:20:00 EST 1997


I think Sigma has a technical bulletin on preparing tris buffers using the base
and the HCl.  Also there are a number of handbooks with buffer preparation
tables.  Check "Data for Biochemical Research" from Cambridge U. Press.

In <344486B7.22BD at ic.ac.uk>, Dr Warren Hirst <w.hirst at ic.ac.uk> writes:
>Does anyone have a table which gives the relative amounts (mass) of 
>Tris-base and Tris-HCl to add to give a 1M solution of various different 
>pH values i.e. 7.4, 8.5 etc.? I am sick of adding a bucket-load of conc. 
>HCl to my 1M Tris-base solution to bring it down to 7.4. I know such 
>biological data tables exist, but as yet I have been unable to locate 
>them. Thank you in advance.
>
>Warren Hirst




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