Dextran sulphate substitute

Karl Fischer tyr-2 at bones.biochem.ualberta.ca
Wed Oct 15 14:24:22 EST 1997


In article <Pine.SOL.3.93.971015114202.10245A-100000 at eukaryota>, Mounir
Izallalen <mounir at biota.rsvs.ulaval.ca> wrote:
 
> I have this question:
> What does the dextran sulphate really do in Hybridization, apart improving
> the results ? I mean it's role. Also, do you know any other cheaper
> substitute for this compound.

I was taught that dextran sulphate in a hyb mix served to reduce the water
volume and, as such, increase the relative concentration of probe.

I seem to recall dextran sulphate being replaced by PEG in some hybs but
don't have a reference handy.

Cheers

karl the hepB guy

-- 
Karl Fischer
tyr-2 at bones.biochem.ualberta.ca





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