DNA degradation

Alexander N. Kukushkin kan at mmcd.cyt.ras.spb.ru
Wed Oct 15 06:01:31 EST 1997


>   Hello!
>   Does anyone know how to inhibit DNase activity? I keep genomic DNA
>   samples at 4 C, and after 2 weeks the DNA seemes to degrade so that
>   it can't be used for PCR etc...has anyone got same kind of problems,
>   i would really need some ideas here! thanks a lot,
>
>   mirkka
>
EDTA binds the cofactors Mg2+ and Ca2+ (to lesser extent) and inhibits many
DNases but not DNase II.
Alex




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