Tris buffer pH

Philip Heacock pheacock at
Thu Oct 16 14:42:54 EST 1997

In article <344486B7.22BD at>, Dr Warren Hirst <w.hirst at> wrote:

> Does anyone have a table which gives the relative amounts (mass) of 
> Tris-base and Tris-HCl to add to give a 1M solution of various different 
> pH values i.e. 7.4, 8.5 etc.? I am sick of adding a bucket-load of conc. 
> HCl to my 1M Tris-base solution to bring it down to 7.4. I know such 
> biological data tables exist, but as yet I have been unable to locate 
> them. Thank you in advance.
> Warren Hirst

Warren try: MIXING TABLE

Philip Heacock, Research Associate      E-mail: pheacock at 
UT-H HSC, Medical School                  Tel: (713) 500-6052                 
Department of Biochemistry                FAX: (713) 500-0652
        and Molecular Biology
6431 Fannin Street
Houston, TX 77030

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