Tris buffer pH

[Philip Heacock]

In article <344486B7.22BD at ic.ac.uk>, Dr Warren Hirst <w.hirst at ic.ac.uk> wrote:

> Does anyone have a table which gives the relative amounts (mass) of 
> Tris-base and Tris-HCl to add to give a 1M solution of various different 
> pH values i.e. 7.4, 8.5 etc.? I am sick of adding a bucket-load of conc. 
> HCl to my 1M Tris-base solution to bring it down to 7.4. I know such 
> biological data tables exist, but as yet I have been unable to locate 
> them. Thank you in advance.
> 
> Warren Hirst

Warren try:
http://www.sigma.sial.com/sigma/techinfo/106b.htm#TRIZMA MIXING TABLE

Phil
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Philip Heacock, Research Associate      E-mail: pheacock at utmmg.med.uth.tmc.edu 
UT-H HSC, Medical School                  Tel: (713) 500-6052                 
Department of Biochemistry                FAX: (713) 500-0652
        and Molecular Biology
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