TCA Precipitation

Morihiro Watanabe watanabe at fcrfv1.ncifcrf.gov
Thu Oct 16 20:39:11 EST 1997


In article <344412D3.429EB6F9 at rascal.med.harvard.edu>,
yip at rascal.med.harvard.edu wrote:

> Does anyone know the lower limit for molecular size peptides which may
> be precipitated by method of TCA precipitation?
> 
> That is, what's the smallest peptides which may be precipitated by TCA?
> 
> thanks in advance folks

I am doing acetate precipitation for small peptide (MW 20kD and 30kD). But
I could not recover the protein after precipitation. It may depend on the
protein but generally which will be better --- TCA, acetone or anything
else?

Thanks

Mori



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