TCA Precipitation

Morihiro Watanabe watanabe at
Thu Oct 16 20:39:11 EST 1997

In article <344412D3.429EB6F9 at>,
yip at wrote:

> Does anyone know the lower limit for molecular size peptides which may
> be precipitated by method of TCA precipitation?
> That is, what's the smallest peptides which may be precipitated by TCA?
> thanks in advance folks

I am doing acetate precipitation for small peptide (MW 20kD and 30kD). But
I could not recover the protein after precipitation. It may depend on the
protein but generally which will be better --- TCA, acetone or anything



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