Maximum binding of proteinA-fusion protein to IgG agarose
jaikawa at UCSD.EDU
Thu Oct 16 19:10:46 EST 1997
Does anyone know the condition for maximum binding of proteinA-fusion
protein to IgG agarose? When I used a spent medium of transfected cells as
a source for the purification, I failed to purify it. Does it need to
adjust pH to the optimal condition for binding to IgG agarose?
Welcome any comments and suggestions.
Jun-ichi Aikawa, Ph.D.
University of California, San Diego
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