Plasmid size limit for transformation

Torben ¯sterlund torben.osterlund at medkem.lumse
Thu Oct 16 12:02:04 EST 1997


I am about to make a construct that will be approx. 21 kb (including the
2.9 kb of the vector, pBluescript).
My question is: will it be possible to transform E. Coli with this
(monster) plasmid  by electroporation?. Should I adopt some special
precautions??? 
 Thanks for your tips!

Juan A. Contreras
Lund University



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