Transfection -Copy number

Steve Cohen sbc4 at PO.CWRU.EDU
Thu Oct 16 10:09:22 EST 1997


Fellow Netters,

I would like to stably transfect my favorites gene into 10T1/2 cells, but I
would like to keep

the number of copies integrated as low as possible. To complicate matters,
I need to cotransfect a 

selectable marker, as the plasmid that contains my favorers gene has no
marker.


Any opinion/ideas/references on methods of choice and ratio of marker/gene
plasmid would be helpful.



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