TCA Precipitation

Peter pxpst2 at vms.cis.pitt.delet.edu
Thu Oct 16 09:36:23 EST 1997


In article <623qhq$2vk_004 at doit.wisc.edu>,
klenchin at facstaff.REMOVE_TO_REPLY.wisc.edu (Dima Klenchin) wrote:

.> In article <pxpst2-1510970948040001 at pelli.pathology.pitt.edu>,
pxpst2 at vms.cis.pitt.delet.edu (Peter) wrote:
.> #The best procedure that
.> #I have found for the recovery of ng  quantities of protein(of any size) is
.> #Phenol/ether extraction.  I have successfully recovered 15 ng from 1 ml. 
.> #TCA cannot do this.
.> 
.> 15 ng/ml... Impressive...
.> Could you post a method or ref.?
.> 
.> - Dima


reference:  Concentration of Dilute Protein for Gel Electrophoresis, Anal.
Biochem., 226, 382-383 (1995)

Peter

-- 
"Don't you eat that yellow snow
    WAtch out where the Huskies go"

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