reverse transcripatase in Taq?

Peter Schuchert schuchert at ubaclu.unibas.ch
Fri Oct 17 16:28:33 EST 1997


It was once reported that Taq can also reverse transcribe RNA.
What's the actual opinion on that?

I try to make rt-PCR of a weakly expressed gene and get specific bands
even without reverse transcription (contamination can be excluded,
the RNA has been treated with DNAseI and the genomic copy has an intron,
so that can be excluded too).
Any hints before I go and try RNAse treatment (reluctantly!!)

cheers,
Peter



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Dr. P. Schuchert
University Hospital INSEL
Gastroenterology Unit  MEM-B814
CH-3010 Bern Switzerland
Tel 0041 31 632 84 92    Fax 0041 31 632 96 65
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