reverse transcripatase in Taq?

Tracy Aquilla taquilla at nospam.erols.com
Sat Oct 18 18:57:32 EST 1997


In Article <3447D881.7FB1 at ubaclu.unibas.ch>, Peter Schuchert
<schuchert at ubaclu.unibas.ch> wrote:
>It was once reported that Taq can also reverse transcribe RNA.
>What's the actual opinion on that?
>
>I try to make rt-PCR of a weakly expressed gene and get specific bands
>even without reverse transcription (contamination can be excluded,
>the RNA has been treated with DNAseI and the genomic copy has an intron,
>so that can be excluded too).
>Any hints before I go and try RNAse treatment (reluctantly!!)

Do a brief search of Medline. You should find several references to Taq's RT
activity.

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