reverse transcripatase in Taq?

Tracy Aquilla taquilla at nospam.erols.com
Sun Oct 19 09:48:35 EST 1997


In Article <344A1471.3487 at mother.com>, Michael Lassner <mikel51 at mother.com>
wrote:
>We tried this out a long time ago.  It didn't work nearly as well as
>doing a separatate reverse transcription reaction.  Personally, I do a
>reverse transcription, purify over an S-400 microfuge spin column and
>then PCR.

This is because the RT activity of Taq only comprises about 10% of the total
activity. RT-PCR can be performed with Taq alone, but I prefer to add some RT.
Tracy



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