lacU169(2nd attempt)

Thomas Eitinger thomas=eitinger at rz.hu-berlin.de
Mon Oct 20 10:23:01 EST 1997


Unfortunately I did not yet receive a reply that really answers the
question whether the lacU169 (lac-argF) deletion also spans lacI. There
are different statements in the catalogs. Life Technologies states that
it spans lacZYA-argF whereas NEB defines it as lacIZYA-argF. Can anybody
help to solve my problem, i.e. whether DH5 alpha (lacU169) produces a
Lac repressor from its chromosomal gene or whether lacI is also deleted.
Thomas



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