reverse transcripatase in Taq?
nospamnishir at ohsu.edu
Mon Oct 20 15:37:07 EST 1997
In article <3447D881.7FB1 at ubaclu.unibas.ch>
Peter Schuchert <schuchert at ubaclu.unibas.ch> writes:
> It was once reported that Taq can also reverse transcribe RNA.
> What's the actual opinion on that?
> I try to make rt-PCR of a weakly expressed gene and get specific bands
> even without reverse transcription (contamination can be excluded,
> the RNA has been treated with DNAseI and the genomic copy has an intron,
> so that can be excluded too).
> Any hints before I go and try RNAse treatment (reluctantly!!)
Is this a gene you have cloned? If you have alot of plasmid encoding
the cDNA flying around, one of your reagents could have gotten
contaminated. Have you tried leaving out a template or testing the
water that you use in any of your reactions? Or the oil (if you use
oil--we have even gotten contaminated oil).
reply to nishir at ohsu.edu
Rae Nishi, PhD
Dept. Cell & Developmental Biology
Oregon Health Sciences University
More information about the Methods