markers on pMC9

Dr. Duncan Clark duncan at
Mon Oct 20 07:00:29 EST 1997

In article <goCYOI600WBN04RlM0 at>, Jeffrey David Bray
<bray+ at> writes
>I have a question from a labmate.  The E. coli strain Y1090 has the
>plasmid pMC9 in it.  What is the drug resistance marker on this plasmid?
> Someone plated Y1090 on Amp and it grew and they wished to know if they
>have made a boo boo.  Thanks for the help.

pBR322 with lacI inserted in EcoR I site so it will Amp and presumably
still Tet.

The problem with being on the cutting edge is that you occasionally get 
sliced from time to time....

Duncan Clark
DNAmp Ltd.
TEl/FAX 01252376288

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