markers on pMC9

Dr. Duncan Clark duncan at genesys.demon.co.uk
Mon Oct 20 07:00:29 EST 1997


In article <goCYOI600WBN04RlM0 at andrew.cmu.edu>, Jeffrey David Bray
<bray+ at andrew.cmu.edu> writes
>I have a question from a labmate.  The E. coli strain Y1090 has the
>plasmid pMC9 in it.  What is the drug resistance marker on this plasmid?
> Someone plated Y1090 on Amp and it grew and they wished to know if they
>have made a boo boo.  Thanks for the help.

pBR322 with lacI inserted in EcoR I site so it will Amp and presumably
still Tet.

Duncan
-- 
The problem with being on the cutting edge is that you occasionally get 
sliced from time to time....

Duncan Clark
DNAmp Ltd.
TEl/FAX 01252376288
http://www.dnamp.com
http://www.genesys.demon.co.uk



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