reverse transcripatase in Taq?
Dr. Duncan Clark
duncan at genesys.demon.co.uk
Mon Oct 20 04:13:05 EST 1997
In article <3447D881.7FB1 at ubaclu.unibas.ch>, Peter Schuchert
<schuchert at ubaclu.unibas.ch> writes
>It was once reported that Taq can also reverse transcribe RNA.
>What's the actual opinion on that?
Taq does have RT activity but it is very weak. Tth has quite a good
level and Tfl is reported to have none. However there appears to be the
odd varient of Tfl around because our sequence data says that the
standard Tfl strain, AT62 pol gene is identical to Tth HB8, the standard
The problem with being on the cutting edge is that you occasionally get
sliced from time to time....
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